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CSSMS provides complete analytical services for the analysis of air, water, industrial hygiene, and biological samples for the OHSU community and external customers. The center also provides for customized method development and data processing services. Customer training is also available for those who find it most effective to use the center instrumentation to do their own sample analysis. All services and instrumentation usage will be charged either on a hourly or as applicable per sample basis. Major instrumentation includes a LECO Pegasus 4D GCxGC –TOFMS system, a PerkinElmer adsorptive/thermal desorption unit coupled with a Finnigan gas chromatography mass spectrometer system (ATD-GC/MS), and a PerkinElmer GC/MS system.
Center Manager
Dr. Wentai Luo, PhD
Phone: 503-748-1090
Fax: 503-748-1464
e-mail: wentai@ebs.ogi.edu
Staff
Lorne Isabelle
Phone: 503-748-1088
Fax: 503-748-1464
e-mail: isabelle@ebs.ogi.edu
Dr. Cai Chen, PhD
Phone: 503-748-1652
Fax: 503-748-1464
e-mail: chen@ebs.ogi.edu
Two-dimensional gas chromatography (GCxGC) uses a pair of distinct stationary phase GC columns (such as a non-polar and polar pair or conversely a polar and non-polar pair) for a single analysis. The first (the longer) GC column provides the primary separation of sample constituents. The second (the shorter) GC column adds a second dimension of chromatographic resolution. The LECO’s Pegasus 4D GC-TOFMS with Leco ChromaTOF uses a 2D GCxGC for chromatographic separation, a high speed acquisition time of flight mass spectrometer for detection, and ChromaTOF software for data acquisition, peak deconvolution as well as component identification and sample quantitation. It provides four dimensions of analytical resolution which is very significant and unparalleled for complete complex sample analysis.
The major applications for the GCxGC-TOFMS system include environmental, food, cosmetic, petrochemical industry and the field of metabolomics. For example, in the food industry, such instrumentation has been used to analyze additives, flavor agents, pesticide residues, and fake commercial products and to evaluate food safety. In the area of metabolomics the instrumentation provides for plant metabolite profiling, biomarker detection, drug safety evaluation and disease detection. Petrochemical industry applications include the analysis of crude oils, biofuels, and finished fuels including additives. The chemical fingerprint profile for an oil contaminated site can provide information for pollution source detection. (application references)
a fuel sample
An engine exhaust extract sample
a contour plot of a cigarette smoke sample
Adsorption/Thermal Desorption Gas Chromatography Mass Spectrometry (ATD-GC/MS)
The ATD-GC/MS system can be used to analyze both air and solid samples. For air samples, an air sample is pulled directly through a glass or metal sampling cartridge that is packed with an appropriate sorbent material. The cartridge is then thermally desorbed and the analytes are transferred to a GC/MS system for separation and detection. For a solid sample, the sample is directly packed into a clean cartridge and thermally desorbed at a chosen temperature.
The ATD-GC/MS system utilizes an OGI developed method for the analysis of volatile organic compounds (VOCs) in indoor, ambient, and unsaturated zone air, as well as industrial hygiene samples. The volatilities of the compounds ranged from that of dichlorofluoromethane
(CFC12) to that of 1,2,3-trichlorobenzene. The OGI VOC method is similar to the EPA TO17 methodology. This system is also used for the analysis of residual VOCs resulting from the manufacturing, pyrolysis or degradation of solid samples such as polymers and natural products on customized method depending on the sample nature. (application references)
An office air sample
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